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ʯīϩҽѧӦãģ

ʯīϩ̼ԭӹɵֻеԭӺȵĶά壬ĹѧѧѧѧѧܡʯīϩĹѧѧѧԣҽҩѧҪӦǰһڣԡʯīϩҽѧӦΪ⣬רռ2020طĿƼϲ㼯ɻṩңϣԡʯīϩҽѧӦȤѺԸ༼𵽽οáڽⷽϽ϶࣬ǽIJṩҡǵIJ֡

 

ʯīϩҽѧӦģ

 

 

34Ǿ-ʯīϩˮڹȱеӦ

ߡٻӨӨղ

ϺѧˮѧҼʵѧʾ Ϻѧѧѧо ϺѧũҵˮˮԴصʵ ϺҽҩѧҽѧԺ ϺҽѧԺҽԺԺǿ

׳ҽѧо  20202

ժҪҽѧķչ,ƱɽҵͶԵˮ𽥳Ϊȱоȵ㡣ǾʯīϩϲϾõԡɽԼڳɹǷֻŵ,ƳɵˮȱչֳܺõӦǰҪ˿Ǿʯīϩ临ˮڹȱеӦ,Ǿʯīϩˮӡ޻ɵิڹȱ͹ǸȾеӦ,԰һЩסҩԼһЩ,:ùء͡BMP-2,Ϊȱ͹޸ṩһµ˼·

 

35ùصĿǾʯīϩˮƱܲⶨ

ߡӨӨٻСղ

Ϻѧ ˮϵҼʵѧʾ/ѧо/ũҵˮˮԴصʵ,ϺҽѧԺ ҽԺԺ ǿ,

׳ϴѧѧȻѧҽѧ棩2020413

ժҪĿ:ƱǾ(CS)ʯīϩ(GO)ˮذù(VAN)ĸʽ΢,ͨⶨʽ΢ȡ̬ҩʺͰʼⶨĿ϶,ѹǿ,ʱ,ɽʱԼ,̽CS-GOϵΪ帺VANʽ΢Ŀ.:ùذڸʽ΢,ȷɢCSGOΪҪԭγɵϵ.ȷDzⶨ΢ƽ,ֹȼƲⶨ΢ҩʡʼеͷ.ͨɨ羵۲˶ߵı̬.:ҩ΢ԺáɢϾȡνϹ,ƽΪ7.07 um,Ϊ(12.200.668),ҩΪ(1.200.007).ɽʱΪ130 s,϶Ϊ(68.243.83),ѹǿΪ(6.3980.295)kPa,ʱɴ41 d,ҩΪ(68.60.028).羵ֲ,϶,ʺϸճ.:CSGOΪԭ,ҩ΢ͬγɾлˮ,ΪһչӦṩ.

 

36ʯīϩЧӦﴫΰϸͷŵ΢

ߡ

ҽҩѧ

׳ҽҩѧ2020˶ʿ

ժҪ΢(MVs)ǴϸԳѿķʽ䵽ϸ⻷еĤ(100-1000 nm),Ŀǰѱ㷺ΪǸְ֢ϷdzӦǰġָơMVsȺĶǴһȫµĽǶϸںϸͨѶ(ת,ں鵼źת)ͬʱ,ͨMVsˮƽ,ԽһMVsγɺDZڵڻ,԰ǸھMVsŨ쳣ķϸԴMVsĸܼڰ֢ҪáȻ,MVsص,ĿǰMVsĸܼڼȻսԡͳMVsⷽ׿ٷ(NTA)ø(ELISA)мȵ͡ƷԼ󡢼ʱ䳤ȱ,ʵٴӦõִ׼Ѹٷչ,ԭʯīϩ(RGO)ЧӦﴫ(FET)Ǽ⡢ȸߡԺáٶȿ졢ƷԼĵ͡Գɱ͡Сͻͼɻŵڸﴫƽ̨ӱݱ,RGO-FETﴫڸӵĸȼ,ᡢʡ񾭵ʡϸлغӵȡȻ,ĿǰʹRGO-FETﴫMVsı,RGO-FETdzϣΪMVs,δٴӦҲйӦǰ״ս,йֹܻRGO-FETﴫֱMVsˮƽĸԼΰϸԴMVs(HepG2-MVs)ĸȡԡǼ,忪չĹ:һֻĤػԵRGO-FETﴫMVsijⱾdzɹһù׺(SA)ܻRGO-FETﴫػԽӦMVsˮƽĸȼ⡣ôƽ̨RGOΪײ,Դͳı׼뵼弼ƱõFETоƬ,ԵͿķʽRGO̶мɵõRGO-FETﴫMVsȱϵͨõױ־,ȶػ(ϸʱػ֬ﲢϸҺļɵõػMVs,B-MVs)ΪRGO-FETﴫʵֶB-MVsļ,б湦ܻ,ͨѧӼ,SAεоƬı档B-MVsϵSAԷԽ,B-MVsԱԵزоƬ档B-MVs,RGO-FETΪp,B-MVsϵоƬʱ,ԶRGOnͲ,źŵĸı(תе˵ı仯)ƽ̨ʵ10~5 particles/mL10~9 particles/mLŨȷΧڵB-MVs,޵20 particles/L,ƽ̨õ,ԱʶԼĸšƽ̨õͨ(ʵֶԶϸԴB-MVsļ)Ϳ(ʵѪB-MVsļ)н,SAܻRGO-FETﴫΪҽѧоMVsˮƽķṩһȫµġɿļԺͼƽ̨ڶ˫幦ܻRGO-FETﴫǡԼHepG2ϸԴMVsоּڹ˫幦ܻRGO-FETﴫ,ӶʵֶԸΰϸMVs(HepG2-MVs)ĸԡȼ⡣,òĵķHepG2ϸҺз봿HepG2-MVsΪʵRGO-FETﴫƽ̨϶HepG2-MVsĸȼ,ƱõRGO-FETﴫ,ûѧԭӦRGOԭλAuNPs,Au-STLS11aEpCAMͬʱεAuNPsоƬ,ʵ˫幦ܻRGO-FETﴫ(AAP-GFET)ĹHepG2-MVs,RGO-FETΪp,HepG2-MVsεоƬ̽Բʱ,HepG2-MVsĸԶRGOnͲ,Ӷѧźŵı仯(תе˵ı仯)AAP-GFETﴫʵֶPBSHepG2-MVsĸȼ,޵84 particles/LAAP-GFETﴫֳõ,ЧΰѪ嵰׺ϸԴMVsĸš,˫ﴫнǿﴫź,Ч˴ļȡ,AAP-GFETﴫӦʵٴѪҺƷHepG2-MVsļ,dzɹϸ(HCC)뽡ֿн,˫幦ܻRGO-FETﴫƽ̨ΪHCCϵߡ

 

37ʯīϩԾۺˮƱ

ߡ޽,̷

ϴѧѧѧԺѧϵ㶫ʡʯīϩ๦ܼܲƷ()̼о

׳20204죩ѧ뼼̳

ժҪˮд̼Ӧ졢Ժáˮˮص,ҩﴫݡ̺֯˿ڷϵҽѧйӦǰ,ۣN-ϩNIPAMˮӽ¶ȵ¶,ʾҩ,Ŀǰѧܲҩ︺͵ȱݶӦá,NIPAMͱϩAAMΪ塢ϩʯīϩGMʯRECΪǿ,ɻۺϷƱһϵNIPAM-AAM/GM/RECˮ,Ȼģҩỷɳǵõ˿ˮ:GMRECļˮѧܺ;ƱĿˮнϸߵҩ︺,37¾ŽȫҩͷĿЧ 

 

38ʯīϩSPRƱԼӼ

ߡϮ̩

ɽʦѧ

׳ɽʦѧ2020˶ʿ

ժҪ˴Ƚϸߡ״ӦԼǿƳ״ĴԵܺáʵԶĿءźԭӷšˮ͸¡͸ʴĻѧŵʹڴҪλȫ䷢ڽĤ֮ӽʱ,ٿŲĴڽ½ɵγɱ塣ٿŲƥʱ,໥֮ᷢ,ᱻĵ,֮Ϊ干(Surface Plasmon Resonance,SPR)干(Localized Surface Plasmon Resonance,LSPR)干Ϊ,,ԱֳĹ׿ȡDz/ʲ㱻Եضѵʱ,޶ڸ弤Ԫĵų,Ӧ,ڹϵĵܹЧشݵ,׽ĵ糡ǿȡ,άṹż,ڴֱܶ,ģʽƽתάṹСDNAӽָͨDNAĿ֮Ŵϵ,֮Ŵһӷڻɸѡͼ,־,ת¼׺ͷֵȷ,DNAӽмҪۼֵʵ塣ĻSPRԼLSPRԭƱSPR˴ԼṹLSPR˴,ҶΪ˼о֮ӼԭƱĹ˴ԼԼӦѧļ⡣,SPRԭSPR˴,ѹӡķ˼ӹDʹ,֮õֱͭʹʯīϩķ,ȻſؽһĤ,ʴͭԻĸϽṹȻ󽫴˽ṹתD͹˴ӶзD͹SPR÷ʹʯīϩ֮ĽӴ,ʹSPRܵͨԱȷ,֤ʯīϩĴڿʹӵиߵ,ҴҺļ,1.33301.3657֮䷢40 nmĺ,֪֮,ƵĴΪ1223 nm/RIUͨݷ֪ôõȶԺظԡ֮LSPRԭ,һά׿ʯīϩĽṹ,ҽӦU͹ƱɶLSPRṹU͹˴оӦγĤ,ַɱ,ȸ,ʱ,,ҼĤӽӴŵ,ʹù˴иߵļȡ֮ûԭʯīϩķһ3 nmʯīϩ,Ĥγɼ㡣ͨԴ˴ĵ干Ϊۺо,ڲĽж,ֵڲΪڲͬʱͬƱڲͬȵĵʯīϩṹ˴,Ա֤ڲṹԵơͬʱҲͨFDTDõģϵ֤Ӽԭ,˴Ʊ˴,ҶԼԼӦѧļ⡣˴,ڲͬŨȵĿDNAеĵ干Ϊۺо,֪Ʊ˴Ը֪˫DNA֮䷢ĺ,ȥDNAļ䡣,˴ķӦѧЩʵ,˴Ϊҽѧ\ļ⿪µ;

 

39ʯīϩΪҩео

ߡȻܰž

ҽѧԺѧѧԺ

׳ҽѧԺѧ  20203

ժҪʯīϩ,Я,нϴıȱõˮԡԡϸߵҩʵŵ,гΪһְȫЧѡԵİҩ塣ʯīϩɸظȻҩ˹ϳɵĿҩҩ,ɽ,Ҫá

 

40ʯīϩڿǻҽѧӦüﰲȫо

ߡ 

ִѧǻҽѧԺֲ

׳ǻҽѧо  20205410-412,3ҳ

ժҪʯīϩԽڿǻҽѧѧǵĹע,о漰ܡɹǷֻ֧֯ܡҩ͵ȷ档ͬʱ,оʯīϩܶϸһ̶ȵ,ﰲȫҲһֱܵǵӡĽ̽ʯīϩڿǻӦ,ﰲȫһ,ΪϵзٴӦṩοݡ

 

41ʯīϩˮзԪʵо

ߡ

йԭܿѧоԺ ѧ˿ѧ빤ѧԺ ʡְҵоԺ

׳лҽѧ־ 20205397-401,5ҳ

ժҪĿоʯīϩ(GO-A)ʵԡˮȻԭװƱGO-A,ɨ羵(SEM)͸Ҷ(FT-IR)б,ԷԷˮʵЧ,ҺpHʱ䡢ʳʼŨȺ¶ȵӰءpHΪ3.0GO-AΪ20 mgҺ25 ml254 hʵ,GO-AʵΪ85.8 mg/gGO-AƱ,Ѻ,Һ뷽,ʾнϸߵ,ΪʷԷˮԼƷǰṩ·¼

 

42ʯīϩԱõо

ߡνʥ骡ʯáľҡ

ѧɽҽԺַԺǻ, 㽭ѧҽѧԺڶҽԺǻ ϺͨѧҽѧԺھҽԺ,Ϻпǻҽѧصʵ,Ϻпǻҽѧо,ҿǻٴо, ϺпǻԺǻڿ ϺмζҽԺǻ йѧԺϺӦо,׳ǻҽѧ20204010

ժҪĿ ̽ʯīϩ(GO?Ag)Ա(S.mutans)ĿüĤγɵ. þγɵλʽϸȾɫGO?AgԸ״̬Ŀ;ýᾧȾɫⶨGO?AgúĤγ.ݲõطDunnett's tͳƷ. ŨGO?AgԸ״̬Ŀͳѧ(P<0.05).ͬŨȵGO?Ag,Ĥγͬͳѧ(P<0.05). GO?AgԱԵĿ,ЧγĤ.

 

43ʯīϩʸϸKOAģ޸õijо

ߡƽųžԶΰ

 ҽҩѧһҽԺ˿ ҽҩѧоԺ

׳ҽѧ־2020 1

ժҪĿоʯīϩ(GO)ʸϸ(UCMSCs)ϥԹؽ(KOA)ģ͵޸áѡ1230ֻ,ֱΪ(ÿ15ֻ):AøHulth+ȱ𷨽KOAģ,Bøľϵøͼע䷨KOAģ͡ÿģΪ4:հ׶(n=3)GO(n=4)UCMSCs(n=4)GO+UCMSCs(n=4)ֱڶģ͵ϥؽǻע0.5 mlŨΪ9 g/LNaClҺGO󻬼(GOŨΪ30g/ml,ܼΪΪ0.25%͸)UCMSCsҺ(510^6/ml)GO󻬼UCMSCsҺ(GOŨΪ30g/ml,UCMSCsŨΪ510^6/ml)øⶨ(ELISA)ƺ8ܸ鶯Ѫһ(NO)ͽԭ(COL-)ǰ(GAG)ϸ-6(IL-6)-(TNF-)ˮƽƺ8,ABGO+UCMSCsѪNOIL-6TNF-ˮƽڿհ׶(P<0.01),ѪCOL-GAGˮƽڿհ׶(P<0.01)Aпհ׶ѪNOˮƽBпհ׶,ͳѧ[(22.0970.352)ng/ml(23.6620.056)ng/ml,P<0.05];AUCMSCsGO+UCMSCsѪCOL-ˮƽֱBжӦ,ͳѧ[(15.5890.063)ng/ml(14.4290.092)ng/ml,(19.3720.063)ng/ml(16.2570.416)ng/ml,P<0.01];Aпհ׶顢GO+UCMSCsѪGAGˮƽֱBжӦ,ͳѧ[(23.8320.891)ng/ml(18.7090.552)ng/ml,(37.4392.155)ng/ml(26.5540.450)ng/ml,P<0.01];Aпհ׶顢GO+UCMSCsѪIL-6ˮƽֱBжӦ,ͳѧ[(16.0820.323)ng/ml(18.3670.861)ng/ml,P<0.05;(7.4260.294)ng/ml(8.6800.242)ng/ml,P<0.01];Aпհ׶顢GO+UCMSCsѪTNF-ˮƽֱBжӦ,

 

44/ʯīϩĺˮĤƱӦ

ߡǿ

йѧԺݻѧо󻬹صʵ йѧԺѧо  ѧѧԺ  ݴѧǻҽѧԺ

׳ ϲѧ, 2021, 38(9):

ժҪ(SA)һȻ߷Ӿۺ(Ag)õĿԣöƱˮҽѧйӦǰȽAg׿ʯīϩ(GO)ƬõAg/ʯīϩ(Ag-GO)ȻٽӵSAУͨܽ-øAg-GO˫㺣ˮĤ(Ag-GO/SA)FTIRXRDSEMȼAg-GO/SAɽṹ΢ò˱ԡԡѧϸԺ˿ܡAg-GO/SAõԡѧǿȺͿԵܣҽɴȣAg-GO/SAɴٽSD˿ϣ˿ʸߴ98%Ϊ˿ڷϾкܴӦDZ

 

45ʯīϩ׸ϲڵ绯ѧеӦ

ߡ νڴ÷

ͨѧѧԺ

׳ѧչ  20213

ժҪ绯ѧ򵥡㡢ȸߡ޵,\ٴ⡢ҽҩҵҪоǰʯīϩ׸ϲʯīϩһЩԲγɵͲϡСߴЧӦҴĵ߻ԼͬܵIJϳõ岢ЭͬЧӦŵ,㷺Ӧڵ绯ѧоʯīϩ׸ϲƱĵ绯ѧҲΪоʵĻѧṩҪϢļҪʯīϩ׸ϲϵ绯ѧķࡢչʷʵƷеӦ,˽ʯīϩ׸ϲڵ绯ѧоչ