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ʯīϩҽѧӦã

ʯīϩ̼ԭӹɵֻеԭӺȵĶάĹѧѧѧѧѧʯīϩĹѧѧѧԣҽҩѧҪӦǰһڣԡʯīϩҽѧӦΪ⣬רռ2020طĿƼϲ㼯ɻṩңϣԡʯīϩҽѧӦȤѺԸ༼𵽽οáڽⷽϽ϶࣬ǽIJṩҡǵ֡

 

ʯīϩҽѧӦ

 

28ʯīϩЧӦﴫΰϸͷŵ΢

ߡ

ҽҩѧ

׳ҽҩѧ - 2020˶ʿ

ժҪ΢(MVs)ǴϸԳѿķʽ䵽ϸ⻷еĤ(100-1000 nm),Ŀǰѱ㷺ΪǸְ֢ϷdzӦǰġָơMVsȺĶǴһȫµĽǶϸںϸͨѶ(ת,ں鵼źת)ͬʱ,ͨMVsˮƽ,ԽһMVsγɺDZڵڻ,԰ǸھMVsŨ쳣ķϸԴMVsĸܼڰ֢ҪáȻ,MVsص,ĿǰMVsĸܼڼȻսԡͳMVsⷽ׿ٷ(NTA)ø(ELISA)мȵ͡ƷԼ󡢼ʱ䳤ȱ,ʵٴӦõִ׼Ѹٷչ,ԭʯīϩ(RGO)ЧӦﴫ(FET)Ǽ⡢ȸߡԺáٶȿ졢ƷԼĵ͡Գɱ͡Сͻͼɻŵڸﴫƽ̨ӱݱ,RGO-FETﴫڸӵĸȼ,ᡢʡ񾭵ʡϸлغӵȡȻ,ĿǰʹRGO-FETﴫMVsı,RGO-FETdzϣΪMVs,δٴӦҲйӦǰ״ս,йֹܻRGO-FETﴫֱMVsˮƽĸԼΰϸԴMVs(HepG2-MVs)ĸȡԡǼ,忪չĹ:һֻĤػԵRGO-FETﴫMVsijⱾdzɹһù׺(SA)ܻRGO-FETﴫػԽӦMVsˮƽĸȼ⡣ôƽ̨RGOΪײ,Դͳı׼뵼弼ƱõFETоƬ,ԵͿķʽRGO̶мɵõRGO-FETﴫMVsȱϵͨõױ־,ȶػ(ϸʱػ֬ﲢϸҺļɵõػMVs,B-MVs)ΪRGO-FETﴫʵֶB-MVsļ,б湦ܻ,ͨѧӼ,SAεоƬı档B-MVsϵSAԷԽ,B-MVsԱԵزоƬ档B-MVs,RGO-FETΪp,B-MVsϵоƬʱ,ԶRGOnͲ,źŵĸı(תе˵ı仯)ƽ̨ʵ10~5 particles/mL10~9 particles/mLŨȷΧڵB-MVs,޵20 particles/L,ƽ̨õ,ԱʶԼĸšƽ̨õͨ(ʵֶԶϸԴB-MVsļ)Ϳ(ʵѪB-MVsļ)н,SAܻRGO-FETﴫΪҽѧоMVsˮƽķṩһȫµġɿļԺͼƽ̨ڶ˫幦ܻRGO-FETﴫǡԼHepG2ϸԴMVsоּڹ˫幦ܻRGO-FETﴫ,ӶʵֶԸΰϸMVs(HepG2-MVs)ĸԡȼ⡣,òĵķHepG2ϸҺз봿HepG2-MVsΪʵRGO-FETﴫƽ̨϶HepG2-MVsĸȼ,ƱõRGO-FETﴫ,ûѧԭӦRGOԭλAuNPs,Au-STLS11aEpCAMͬʱεAuNPsоƬ,ʵ˫幦ܻRGO-FETﴫ(AAP-GFET)ĹHepG2-MVs,RGO-FETΪp,HepG2-MVsεоƬ̽Բʱ,HepG2-MVsĸԶRGOnͲ,Ӷѧźŵı仯(תе˵ı仯)AAP-GFETﴫʵֶPBSHepG2-MVsĸȼ,޵84 particles/LAAP-GFETﴫֳõ,ЧΰѪ嵰׺ϸԴMVsĸš,˫ﴫнǿﴫź,Ч˴ļȡ,AAP-GFETﴫӦʵٴѪҺƷHepG2-MVsļ,dzɹϸ(HCC)뽡ֿн,˫幦ܻRGO-FETﴫƽ̨ΪHCCϵߡ

 

29ӦʯīϩPLCL/Gelɴ֧ܲϵĿԷ

ߡѩΰձٻ̷Ī÷

ϺͨѧҽѧԺϺͯҽѧ ѧѧ﹤ѧԺ֯ʵ ϺͨѧҽѧԺϺͯҽѧתҽѧо 

׳֯ؽ־2020 2 

ժҪĿ ̽Ӧʯīϩ(Gr)-L--/(PLCL/Gel)ɴ֧ܲϵĿ. Ƶľ˿װ÷ֱƱPLCL-GelGr-PLCL-Gelˮɴ֧ܲ.ʹ΢,ɨ΢۲֧̬ܲṹ;ͨ۲òϻеǿ;CCK-8ʵ֧,ֲ֧Ƥ,۲ϸ. ʾ,ʯīϩ֧ܵѧ.֧ܶϸ,ʵоֳõ,ʵʯīϩһ̶ϼ֢Ӧ. ӦʯīϩPLCL/Gelɴ֧ܲ,Իþкѧ,ˮ,Եɴ֧ܲ,֯ƤȷӦDZ.

 

30лܻʯīϩƱӦ

ߡ԰ҦԶ

ѧԺӦûѧ뻷

׳˴ѧԺѧ

ժҪʯīϩһ͵Ķάƽײ,еĵԭӲṹʹӵ,2004,˿ѧĸ߶,ĿǰѳΪ˲ѧ,ѧ,ѧѧоȵ.Ȼʯīϩž۶ѻʯī,ܾȵķɢڻ,̶ܴӦ.Ϊ˽ʯīϩЧ,ַڵѧ,ҽѧ,߻,,ܵ,йܻЧķ֮һ.ؽʯīϩлܻƱӦõоչ,ʯīϩĹܻչչ.

 

31ӦDNAӵ̼׹ܺʯīϩĤго

ߡ

Ŵѧ

׳Ŵѧʿ

ժҪǺᣨdeoxyribonucleic acid,DNAҪ,ǴŴʡDNAӵӽٴҽѧ϶ڼϡͻԼҪ塣Ŀǰ,Թѧ⡢ѧ͵绯ѧΪޱǼDNAӵķΪбǼٶܵ㷺о,ѧⷽʽ,ɱϵ,ǵǰһֱȽձIJԷ̼ײе̼׹ܣcarbon nanotube,CNTʯīϩgrapheneֳѵĵѧе,΢гľѲϡǶصԭӽṹӽ,dzﴫСԴΪijЧӦܱDNAӽȻ÷ڲװõĸ,ʵ޷ЧظԵ⡣ڴ,Ľ΢гﴫн,̽о̼׹ܺʯīϩ΢г̽ⵥDNAҺŨȡͳ΢гǹƬ,뵼ʹùڽϴļ,ڸƵMHzϴ,ŲԾȡòƬԶ׼̹΢гȡͳĹƬ,֮òƬļ,ΪԱ,ͬʱͬµĹƬļݡԽ,Զ׼Ϊ50fF,ͬĹȴﵽ2 pFýӾķ̼׹װ,γ˹̶мյ̼׹ܱĤΪ֤ѧ,ð뵼Dzྦྷ,ߺתߡŴӼDNAŨȵIJϵͳ,ʸǺǰ÷ŴϵS21,Դʵʱμеĵ̼׹ܱĤϵĵDNAҺŨ,ȡϵS21ֵӦƵΪгƵʡ״뵥̼׹ܱĤϵ̽뵥DNAŨȻӰгƵ,νƵʺơ̽뵥DNAҺŨȵ,гƵƫ󡣾̽뵥DNAӱʹ̼׹ܱĤгٴβ֮ԵĵDNAʱ,ʾ֮ǰ෴,гƵʳνơŻԵĵDNAҺŨȵ,гƵʵƫҲʵʱʱһ60֮,ҿԻõDNAŨȲԼΪ5 nMol/LʵӦ̼׹̼׹ܱĤĵ䴿Ⱥͷ,ʯīϩΪά,һ̶ֲ̼׹ܵIJ㡣̼׹ԽǵƶΪ뵼ͺͽ,ӶӰ̼׹ĵѧ,Ŀǰķ뼼ѻøߴȵĵһ͵̼׹ܡ,̼׹ܵļõŹܵķ,ʵе̼׹ܱĤʵָ߶ȵķһԡЩ̼׹IJܡʯīϩɵ̼ԭɵľз״νṹ,Ҳ̼׹һֳǿĵͻеܡʯīϩˮеķɢԲ,׳ž,ҺͨӾķʽװ,ýӾķʯīϩװΪ,γ˫˹̶мյıĤڵ£200Ĺʽ¯,ϵʯīϩĤԭλԭɨ΢ԭ΢ʯīϩò,XǺDZԭǰʯīϩ,˻ԭʯīϩĤĵѧ,200,ϵʯīϩĤĻԭЧѡͬIJϵͳʯīϩĤ΢гDNAӵļ̽뵥DNAʯīϩĽϻôϵS21ɨƵʵĹϵ߳,Ũȴ5Mol/L½5nMol/Lʱ,ķֵӦгƵʺ120 kHz0̽DNAӱʹʯīϩһԵDNAŨʱ,ϵS21ɨƵʵĹϵơDNAŨΪ50 nMol/Lʱ,ķֵӦгƵΪ10 kHzս̼׹ܱĤʯīϩĤIJԽжԱ,̼׹ܱĤIJԾȽϸ,ظԽϲ,ʯīϩĤг෴ܿƱͲϵйءܵ˵,̽һ΢ͱĤгʵʱټⵥDNAŨȵʵ÷,ΪԺʵʵʱԭλһĹס

 

32ڽӺʯīϩĵĤо

ߡ  

人֯ѧ

׳人֯ѧ2020˶ʿ

ժҪŵ˿ڵӺͲԴIJ,ԿԴھ˿йߵĹ㷺עԴԴ̫,˷չ̫ܸЧüҪ塣תǶ̫ܸЧõһЧֶ,йЧӦIJϿɽ̫תΪ,Ӷʵ̫ܵռáڹת,ӺʯīϩĹʡӾĹѧ-干(LSPR)LSPRЧӦܽ״ߴΧʵӰ졣ҲͬɳɷֵĹҲֳͬLSPRģʽ,չֳԲͬεĹաڹתӦľ͹ǽӡάʯīϩнϺõĵЧ,ͬʱкܸߵĹתЧ,Ҳ̫µˮĹתĤƱӦáӺʯīϩһܹþǿתܵĸϲ,ʵӦ÷ΧĤΪһַȻʲ,صάά״Ĥṹӵ塣ּھάάṹĵĤϸϾйתʵĽӺʯīϩƬ,ǿЧӦĸĤ;оڵĤϵĸװʽ,ʯīϩЭͬתЧӦ,ΪԵĤΪĹԸĤڹҩͷŵDZõ춨Ҫ:1.̫¸ؽӺʯīϩϵĤĹоͨ-ʯīϩƬڵĤ档õĤĻԭ,ˮԡȵķڵĤԭλɽӡͨʯīϩĸԼӵԭλϳ̼ɻý/ʯīϩ/ĤԸĤò뻯ѧṹбһ˸ĤĹ,ϵͳ˲ͬƷı¶ʱı仯,ԲͬĤĹϸطڵһӸԵĵĤ,/ʯīϩ/ĤϽṹԵǿתЧӦ2.̫¸ӺʯīϩĸϵĤĹо,ΪǰͨյϳƱضLSPRʵӡװķϳɵװĤ,ͨ-ʯīϩƬװӵĵĤ,Ʊ/ʯīϩ/Ĥ˲ͬĵĤƷĹѧ,òԼѧɡͬڽ,LSPRʸƷصĹϸ˲ͬĤƷĹܡ/ʯīϩ/ĤĤǿĹתܡƱĹתϲϿҽѧĹкܴӦǰ

 

33ʯīϩֹ˹դLSPR̽ﴫо

ߡǽ

ѧ

׳ѧ2020˶ʿ

ժҪ,ڹ˹դľ干ЧӦ(LSPR)Ĵҽѧﻯѧõ˹㷺оӦáǹ˹դ(ExTFG)ڴͳIJդ(FBG)ͳڹդ(LPFG)м͵¶ȽЧӦ,ڶȶҪ󼫸ߵ߷Ӧ/ơͶάײʯīϩ(GO)ھзḻĺšȱص,ﴫı,﹦ܻЧ,ﴫļܡĿǰGOExTFGﴫر,ҴΪ͸ʽ,ʵԭλ⡣ExTFGΪƽ̨GOεLSPR̽ʽﴫ,ڶԸΰ־ϸ-1(PD-L1)ӵļ,Ҫо:(1)ϸExTFGģ,ExTFGλƥģʽԭ,ӦLSPRǿExTFGܵĻ۷,Ϊʵ춨ۻ(2)GOɵExTFG-LSPRﴫ,PD-L1ԭӽм,Ѫ廷²ⶨͷٴԡȽߴ׿(AuNS)εExTFGExTFG-LSPR,GOΪܻϳڴ,PD-L1¡ΪĿʶԪ,ǿԡиβ趼ͨ۽Ϸʽ,ʹӵиߵȶԡڳ·ɨ羵(FESEM)¶Թ˵ıμܻЧԽм׷ԲͬŨȵPD-L1м,,,GOɵExTFG-LSPRߴPD-L1ļⷶΧΪ0.038-38.46pM,0-1.92pMĵŨõԶ(R~2=0.927),ȴ~0.114nm/pM,⼫(LOD)Ϊ~0.076pM,PD-L1ѭLangmuirģ,ϵΪ~2.80110~(-12)M,ʹ(HF)Һ΢ʴդ,ͬηGOɵExTFG-LSPRﴫ,ֱ齡ѪΰѪм,ߴڽѪг񲨳仯С,ڸΰѪг񲨳~0.42nm,֤õԼٴӦõDZ(3)ʵGOɵExTFG-LSPRﴫ̽ṹ,PD-L1м,ԽмExTFG̽ṹƼʵϵͳ,ʵʱ۲졢ɼݡʹûԭ̽,ӷ,ʹëϸܶԶзװ,ǿȶԡʹͬıκ﹦ܻδ,PD-L1м⡣,̽ʽGOExTFG-LSPRﴫPD-L1ļⷶΧΪ0.038-38.46pM,0-1.92pMĵŨ򱣳õԶ(R~2=0.91),ϵΪ~2.82510~(-12)M,͸ʽͬ̽ʽڶ,ڵŨļ~0.127nm/pM,~11.4%,⼫~0.071pM,͸ʽõ˽һơ