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ʯīϩҽѧӦã

ʯīϩ̼ԭӹɵֻеԭӺȵĶά壬ĹѧѧѧѧѧܡʯīϩĹѧѧѧԣҽҩѧҪӦǰһڣԡʯīϩҽѧӦΪ⣬רռ2020طĿƼϲ㼯ɻṩңϣԡʯīϩҽѧӦȤѺԸ༼𵽽οáڽⷽϽ϶࣬ǽIJṩҡǵڶ֡

 

ʯīϩҽѧӦ

 

16ʯīϩҵԱķι

ߡʯ

ʡͶְҵоԺ

׳лͶְҵ־20206

ժҪĿ˽ijҵʯīϩҵԱְҵ,Ϊְҵṩݡ20199,ռ201918Ժְҵijʯīϩҵ54Ӵʯīϩ۳ҵԱ,䡢ķι

Ĺϵ,2ȽָIJ졣ҵʯīϩҵԱ54,ְҵ쳣15,27.8%,Ĺ쳣7,ι쳣8͹,Ĺι쳣(2=0.042,P<0.05)ʯīϩз,ԱӴ뱩¶ʯīϩĻ,ּ˽ʯīϩԹԱ彡Ƿв,ΪְҵԤṩݡ

 

17ʯīϩҩϵƱϸɱ

ߡžǾ

ѧ

׳Ӧûѧ, 2021, 38(6):

ժҪ ͨҶ(PEG)֧;ϩǰ(bPEI)ʯīϩ(NGO)Ϊҩ׵ҩƽ̨,NGOˮԼԵ׵,ֱؿҩ˳(CDDP)͵ҴᴿѪγܹԸڱʰϸĴʯīϩҩϵͨ-ɼ׺͸Ҷ任ױ֤ʵNGO-PEG-bPEI-CDDPҩϵƱɹ,NGO-PEG-bPEICDDPҩΪ34.6% ۱ϩӾ(SDS-PAGE)NGO-PEG-bPEI-CDDP-AntibodyҩϵԿ嵰׵ǿ,׸ܹԵظϸλ,˱ʰϸ(CNE-1)ϸϵʶСϸʵ(MTT)ʵ:ͬ(Ũ)ͬʱ,NGO-PEG-bPEI-CDDP-Antibodyʯīϩҩϵ߶˿ǻ۰ϸ(KB)CNE-1ɱúͱϸĶˡ NGO-PEG-bPEI-CDDP-Antibodyҩϵܹͨʶҩ︻ڲϸ,ܹЧɱ˰ϸ,ͻҩʹü,һֺǰĴҩϵ

 

18ʯīϩ︴ϲ֯еӦ

ߡԪܡٻơҶľáɳ

½ҽƴѧڶҽԺǻ

׳ҽѧ־2020,41(11)

ժҪʯīϩҽѧ򷢻ӽϴ,֯,ҩ,ƵȷӦҪо.оõ,ٽϸɹǷֻϸԵ,֯Ӧһ.

 

19ʯīϩײϵﰲȫоչ

ߡݷԽ

йѧԺ̬޸صʵ,йѧԺ̨о

׳³ѧѧ2020, 36(1)

ժҪʯīϩײϾкܶص,㷺ӦԴϡҽѧ.ʯīϩϵĴģ͹㷺Ӧ,䲻ɱرŷŵ,ͻDZڵΣ,ʯīϩﰲȫܵ㷺ע.Ȼкܶ౨ָʯīϩײϾõ,доһﶾ.ڴ,˽ʯīϩײϵĻΪﶾԺ϶Եоչ,Ϊʯīϩײϵﰲȫṩ.

 

20ʯīϩյϸɹǷֻоչ

ߡƳɭܺΰ

е»ְҵѧԺ ɽҽԺǻŴѧ谲ҽԺǻ

׳¿2020(000),003

ժҪʯīϩȫϵ,ǿ͵һά;,,ѧ,мΪӦǰ.Եʯīϩҽѧҩ,ϸ,,Ƶȶֹ,ǶɹǷֻо,ȡijɹ.ĶԽʯīϩ,ֿͬռṹĶԱȼյϸɹǷֻ,Ϊ֯Ӧ.

 

21ʯīϩ׿γڹդߴ

ߡʯʤ ²   ־ ޱ   

ѧ˴صʵ

׳ѧѧ 202018

ժҪһֻʯīϩ׿(EGO-AuNS)γڹ˹դ(LPFG)ߴ,Ӧв(AIV)⡣þԭʯīϩ(GO)ڽ׿(AuNS)γEGO-AuNSϲ;ùżԹۼϷʽ̶LPFG;AIV¡(AIV-MAbs)ΪʶԪ,̶ڹդ湹EGO-AuNS-LPFGߴ:(RI)1.333--1.411Χ,EGO-AuNS-LPFGRIȸߴ-66.60 nm/RIU,ԼΪͨLPFG6ͨԲͬŨȵȼAIVԭҺļ,ߴļ⼫ԼΪ8 ng/mL,͵ԼΪ50g/mL,ӦļԼΪ2946.25 pm/(gmL^-1),ԼΪGOͿ㸯ʴͨLPFGAIVߴȵ7.3;,AIVӵĽϵԼΪ3.4910^-9 mol/LͨԼҺĶռʵ,ߴҾõԺٴЧ,ҽѧнϴӦDZ

 

22ʯīϩƬСϸЧӦĵ

ߡtȨܻ

ѧҽѧѧԺͶ뻷ϵ

׳ְҵҽѧ2020375

ժҪ[]ʯīϩ(GO)㷺Ӧѧ.о,GOܹϵͳĵڹ,ЧӦؾر.[Ŀ]̽GO֬(LPS)Ͱϸ4(IL-4)յСϸM1M2ѡתе񾭵.[]GOͶ϶BV2ϸ,ֱ20 gL-1 LPSIL-4յBV2ϸת,Ϊհ׶顢GO顢հ-LPS顢հ-IL-4GO-LPSGO-IL-4.Ƚ24hеϸ.ʵʱӫⶨPCR (RT-qPCR)ʽϸֱBV2ϸM1ͱ־[(TNF-)յһø(iNOS)ϸCD86]ԼM2ͱ־[˾ø1(Arg1)ϸ-10 (IL-10)ϸCD206]ı仯,ͬʱʹ÷ֹȷһ(NO)ĺ.[]հ׶,GOBV2ϸϸ(218.138.77175.638.24,P0.01).ڿհ-LPS,GO-LPSBV2ϸM1ͱ־TNF-iNOSmRNAԱ(8.160.265.350.42;45.642.0330.391.84)ԼCD86ϸ[(14.650.85)(10.290.46)],NOĺ[(19.621.07) molL-1(8.371.25) molL-1],M2ͱ־IL-10mRNAԱ(1.630.092.090.15) (P0.05).հ-IL-4,GO-IL-4M1ͱ־iNOSmRNAԱ(0.410.031.050.07),M2ͱ־Arg1ԼIL-10mRNAԱ½(201.6312.314.740.38;2.630.140.710.13),CD206ϸʺƽӫǿ½[(17.011.03)(3.660.41);8893.2347.621299.9159.67] (P0.01).[]GOBV2Сϸ,LPSIL-4յµЧӦBV2СϸM1M2͵ת.

 

23ʯīϩֲӦеоչ

ߡĵ

ִѧǻҽԺǻ޸

׳ǻҽѧо20203611

ժҪʺõԱ㷺Ӧֲ͹ǿֲ,ֲԿֲܷʧܻ߷ֲΪĵĸȾ.ΪŻֲ,ѱѧѾΪоȵ.ʯīϩõĻеܡǴԡյԡԡԡ׹ܻص,ĿǰԽԽرӦڸϵз.ĽʯīϩڽѱоеӦ.

 

243Dӡʯīϩǿϲоչ

ߡǵǺӢŽ

бѧϿѧ빤ѧԺ

׳ϵ2020, 34(9)

ժҪ,ʯīϩѧѧԼģĿ,ʹΪоȵ.ʯīϩΪǿӵۺPմɵȲ,߸ϲϵۺ.ԸϲŻ,Ʊʯīϩǿϲϵ췽Ӧ˶.,3Dӡ߹ƱIJƷŵ,ѧߵĹ㷺ע.Ȼ,ʯīϩܶȵ͡ȱž,ԸϲϵѧܻɲӰ,մɻʪԲ,޷γõĽ.,νʯīϩȷɢڸϲһֱѧǵоص,ĿǰķɢҺϷϷԭλϷͻеĥ.,ʯīϩɢڽմɻеķҪҺϺͻеĥ,ʯīϩȷɢڻв߸ϲϵѧ,ܸĽ.о϶ʯīϩǿۺ︴ϲϻϷҪҺϡϺԭλϵ,õάƷѧܡȺ͵ܾнϴ,Ӧҽѧֳõѧ.Ĵӽϲϡۺϲϼմɻϲ3Dӡʯīϩǿϲϵоչ,ƷۺͳǶϸʯīϩǿϲ3DӡӦ.ڴδʯīϩǿϲϿԹ㷺ӦԴ֧ܼ캽յ,ΪϵоչĿռ.

 

25ӦʯīϩPLCL/Gelɴ֧ܲϵĿԷ

ߡѩΰձٻ̷Ī÷

Ϻ ϺͨѧҽѧԺϺͯҽѧ  ѧѧ﹤ѧԺ֯ʵ

׳֯ؽ־2020162

ժҪĿ ̽Ӧʯīϩ(Gr)-L--/(PLCL/Gel)ɴ֧ܲϵĿ. Ƶľ˿װ÷ֱƱPLCL-GelGr-PLCL-Gelˮɴ֧ܲ.ʹ΢ɨ΢۲֧̬ܲṹ;ͨ۲òϻеǿ;CCK-8ʵ֧,ֲ֧Ƥ,۲ϸ. ʾ,ʯīϩ֧ܵѧ.֧ܶϸ,ʵоֳõ,ʵʯīϩһ̶ϼ֢Ӧ. ӦʯīϩPLCL/Gelɴ֧ܲ,ԻþкѧܡˮԡԵɴ֧ܲ,֯ƤȷӦDZ.

 

26ʯīϩײϵﰲȫоչ

ߡ

йѧԺ̬޸صʵ,йѧԺ̨о,

׳³ѧѧ2020, 36(1)

ժҪʯīϩײϾкܶص,㷺ӦԴϡҽѧ.ʯīϩϵĴģ͹㷺Ӧ,䲻ɱرŷŵ,ͻDZڵΣ,ʯīϩﰲȫܵ㷺ע.Ȼкܶ౨ָʯīϩײϾõ,доһﶾ.ڴ,˽ʯīϩײϵĻΪﶾԺ϶Եоչ,Ϊʯīϩײϵﰲȫṩ.

 

27ʯīϩʸϸKOAģ޸õijо

ߡƽųžԶΰ

ҽҩѧһҽԺ˿  ҽҩѧоԺ 

׳ҽѧ־2020 1 

ժҪĿоʯīϩ(GO)ʸϸ(UCMSCs)ϥԹؽ(KOA)ģ͵޸áѡ1230ֻ,ֱΪ(ÿ15ֻ):AøHulth+ȱ𷨽KOAģ,Bøľϵøͼע䷨KOAģ͡ÿģΪ4:հ׶(n=3)GO(n=4)UCMSCs(n=4)GO+UCMSCs(n=4)ֱڶģ͵ϥؽǻע0.5 mlŨΪ9 g/LNaClҺGO󻬼(GOŨΪ30 g/ml,ܼΪΪ0.25%͸)UCMSCsҺ(5106/ml)GO󻬼UCMSCsҺ(GOŨΪ30 g/ml,UCMSCsŨΪ5106/ml)øⶨ(ELISA)ƺ8ܸ鶯Ѫһ(NO)ͽԭ(COL-)ǰ(GAG)ϸ-6(IL-6)-(TNF-)ˮƽƺ8,ABGO+UCMSCsѪNOIL-6TNF-ˮƽڿհ׶(P<0.01),ѪCOL-GAGˮƽڿհ׶(P<0.01)Aпհ׶ѪNOˮƽBпհ׶,ͳѧ